Gene transfer into vascular cells
نویسندگان
چکیده
منابع مشابه
Liposome-mediated gene transfer into human vascular smooth muscle cells.
BACKGROUND Complexing recombinant DNA with cationic liposomes is a convenient means of introducing foreign genes into cells (lipofection) and could potentially form the basis for genetically modifying diseased blood vessels in patients. The mechanism of lipofection is incompletely understood, but it is recognized that the degree of successful gene transfer is highly dependent on cell type. To d...
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The electric field method of gene transfer via electroporation of cell membranes is gaining importance in cellular and molecular biology. In particular the electroporation technique provides the low copy number transfection required fqr genetic studies. As demonstrated with mouse lyoma cells, with lymphoid cell lines and with plant protoplast, the electric method has appreciable advantages comp...
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This article reviews 1) the use of gene transfer methods to genetically manipulate hematopoietic stem cell targets, 2) recent advances in technology that are addressing problems that have prevented widespread successful translation of gene transfer approaches for the cure of disease, and 3) recent regulatory issues related to human gene therapy trials. In Section I, Dr. Nienhuis describes the u...
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A new field of gene transfer is emerging as a simple, effective means to drive the expression foreign genes in cells: ultrasound-mediated gene transfer or sonoporation. We report here that sonoporation is an effective means of gene transfer for cultured neurons, a cell type that has been difficult to transfect. Neuronal cell types that are effectively sonoporated include chick retinal neurons, ...
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18. Simons M, Rosenberg RD. Antisense nonmuscle myosin heavy chain and c-myb oligonucleotides suppress smooth muscle cell proliferation in vitro. Circ Res. 1992;70:835-843.19. Biro S, Fu YM, Yu ZX, Epstein SE. Inhibitory effects of antisenseoligodeoxynucleotides targeting c-myc mRNA onsmooth musclecell proliferation and migration. Proc Nadl Acad Sci U S A.1993;
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ژورنال
عنوان ژورنال: Journal of the American College of Cardiology
سال: 1991
ISSN: 0735-1097
DOI: 10.1016/0735-1097(91)90957-b